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Rabbit anti-calretinin

7697-200 µl.
货号 货期 规格 / 价格 询价

Rabbit anti-calretinin

品牌

Swant

货号

7697-200 µl。

产品分类

Antibodies against Ca2+ -bindin

研究领域

Calretinin

产品概述

After reconstitution freeze in small aliquots (e.g. 1 µl) and keep at - 80°C (or at least - 20°C). For continuous use, keep at 4°C (with 0.01% Na-azide). Avoid repeated freezing and thawing.

形式

The antiserum against calretinin is produced in rabbits by immunization with recombinant human calretinin containing a 6-his tag at the N-terminal. The antibody was evaluated for specificity and potency: a) by Biotin-Avidin labeling of cryostate-, vibratome- and paraffinsections of 4% paraformaldehyde fixed brains and b) by immunoenzymatic labelling of immunoblots. The product is a polyclonal antiserum against calretinin (1), a calcium-binding protein of the EF-hand family related to calbindin D-28k and calmodulin. The antibody reacts specifically with calretinin in tissue originating from human, monkey, rat, mouse, guinea pig, chicken and fish (Fig. 1). This antiserum does not cross-react with calbindin D-28k or other known calcium binding-proteins, as determined by immunoblots and by its distribution in the brain (Fig. 2).

浓度

Calretinin

纯度

Rabbit

试剂准备

Lyophilized whole serum (no preservatives).

复溶

1. Schwaller B., Buchwald P., Blümcke I., Celio M.R. and Hunziker W. (1994) Characterization of a polyclonal antiserum against the purified human recombinant calcium-binding protein calretinin. Cell Calcium 14: 639-648. 2. Schiffmann S.N. et al (1999) Impaired motor coordination and Purkinje cell excitability in mice lacking calretinin. PNAS, 96: 5257-5262. 3. Gotzos V., Vogt P. and M.R. Celio (1995) Calretinin is a selective marker for malignant pleural mesotheliomas of the epithelial type. Pathol. Res. Pract. 192:137- 147. 4. Doglioni, C. et al. (1996) Calretinin: a novel immunocytochemical marker for mesothelioma. Am. J. Surg. Pathol. 20:1037-1046.

应用

Immunohistochemistry: 1:2'000 - 1:5'000, on paraformaldehyde (4%) or formalin-fixed tissue, cryostate or paraffin-sections. Immunoblots: 1:1'000 - 1:2'000. For immunohistochemistry and immunoblots the titer was determined by using the avidin-biotin method. We recommend that the optimal dilutions be determined by titration experiments.

产品描述

Lot No: 1893-0114 Reconstitution: with 200 µl of bidistilled water. Immunoblot: In Fig. 1, extracts of soluble proteins were isolated from whole brains of different species and separated by SDS-PAGE. In the Western blot the antiserum 7697 specifically recognizes a band of 29-30k Da.Fig. 1 Immunoblot of brain homogenates of various species with antiserum 7697. 1: Mouse, 2: Rat, 3. Guinea pig, 4. Rabbit, 5.Macaca fascicularis, 6. Zebrafish, 7. Chicken, 8. recombinant calretinin Immunoblot of brain extracts: Antibody 7697 labels a subpopulation of neurons in the normal brain with high efficiency (Fig. 2a), but does not stain the brain of calretinin knock-out mice (Fig. 2b). Product: Antiserum uses: Calretinin belong probably to the class of “trigger” calcium binding-proteins. It occurs mainly in subpopulations of nerve cells and is an excellent markers for mesotheliomas(3,4).

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